International Journal of Modern Biochemistry
نویسنده
چکیده
Application of green fluorescent protein (GFP) as a molecular biosensor or reporter is limited by several factors especially its poor fluorescence at low pH conditions. There are conflicting data on this limitation, however, systematic studies to assess the importance of this factor for growing bacterial cultures are lacking. In this study, effect of low pH on GFP green and red fluorescence intensities was demonstrated in batch cultures of Escherichia coli SCC1. The bacterial cells that express GFP were grown and photoactivated with blue light which made them absorb green light and emit red. In order to assess the fluorescence intensity under different pH conditions, relative fluorescence (RF) (absolute fluorescence/OD650) and percentage red-fluorescent to green (R/G (%)) were determined aerobically and anaerobically. The green and red fluorescence intensities were found to be reduced in pH values 4.0 and 5.0. The GFP green fluorescence has very low intensity of less than 70 RFU at pH 5.0 and about 20 RFU at pH 4.0. The intensity was shown to be higher (up to 110 RFU) at pH 6 7 in the aerobic cultures. It was shown that GFP red fluorescence changes with time in different pH conditions both aerobically and anaerobically. The red fluorescence intensity has higher magnitude anaerobically compared to the aerobic cultures. Thus, at pH levels 4 5, the red and green fluorescence intensities were both found to be significantly reduced.
منابع مشابه
Zinc Finger 259 Gene Polymorphism rs964184 is Associated with Serum Triglyceride Levels and Metabolic Syndrome
Metabolic syndrome (MetS) is characterized by a cluster of cardiovascular risk factors that include: abdominal obesity, dyslipidaemia, hypertension, insulin resistance and impaired glucose tolerance. Recent genome wide association studies have identified several susceptibility regions involved in lipid metabolism that are also associated with MetS. We have explored the association of 9 genetic ...
متن کاملThe 15th Iranian National Congress of Biochemistry & 6th International Congress of Biochemistry and Molecular Biology
متن کامل
On the Free Energy That Drove Primordial Anabolism
A key problem in understanding the origin of life is to explain the mechanism(s) that led to the spontaneous assembly of molecular building blocks that ultimately resulted in the appearance of macromolecular structures as they are known in modern biochemistry today. An indispensable thermodynamic prerequisite for such a primordial anabolism is the mechanistic coupling to processes that supplied...
متن کاملInteractive computer simulations of genetics, biochemistry, and molecular biology.
This article describes three interactive software simulations targeted to key domains of modern molecular genetics: genetics, biochemistry, and molecular biology. These simulations allow students to deepen their understanding of key principles in these domains by setting up crosses, designing proteins, and designing genes; the simulations then apply these principles to produce results that the ...
متن کاملThe role of nutrition in the management of lower extremity wounds.
From 3000 BC, healers have understood the importance of nutrition in wound care and used nutritional supplements to aid healing. Many of the teachings from Hippocrates, the father of modern-day medicine, clearly state his firm belief that good health can be achieved through nutrition. In the early 1900s when advances in biochemistry were frequent, many of the essential constituents of nutrition...
متن کامل